PT - JOURNAL ARTICLE AU - Abigail Holborow AU - Hibo Asad AU - Lavinia Porter AU - Poppy Tidswell AU - Claire Johnston AU - Ian Blyth AU - Alice Bone AU - Brendan Healy TI - The clinical sensitivity of a single SARS-CoV-2 upper respiratory tract RT-PCR test for diagnosing COVID-19 using convalescent antibody as a comparator AID - 10.7861/clinmed.2020-0555 DP - 2020 Nov 01 TA - Clinical Medicine PG - e209--e211 VI - 20 IP - 6 4099 - http://www.rcpjournals.org/content/20/6/e209.short 4100 - http://www.rcpjournals.org/content/20/6/e209.full SO - Clin Med2020 Nov 01; 20 AB - The clinical false negative rate of reverse transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 on a single upper respiratory tract sample was calculated using convalescent antibody testing as a comparator. The sensitivity in symptomatic individuals was 86.2% (25/29). Of the missed cases, one (3.5%) was detected by repeat RT-PCR, one by CT thorax and two (7.1%) by convalescent antibody. The clinical false negative rate of a single RT-PCR on an upper respiratory tract sample of 14% in symptomatic patients is reassuring when compared to early reports. This report supports a strategy of combining repeat swabbing, use of acute and convalescent antibody testing and CT thorax for COVID-19 diagnosis.