PT - JOURNAL ARTICLE AU - Ashwin Krishnamoorthy AU - Subashini Chandrapalan AU - Gohar JalayeriNia AU - Yaqza Hussain AU - Ayman Bannaga AU - Ian Io Lei AU - Ramesh Arasaradnam TI - Influence of seasonal and operator variations on diagnostic accuracy of lateral flow devices during the COVID-19 pandemic: a systematic review and meta-analysis AID - 10.7861/clinmed.2022-0319 DP - 2023 Mar 01 TA - Clinical Medicine PG - 144--150 VI - 23 IP - 2 4099 - http://www.rcpjournals.org/content/23/2/144.short 4100 - http://www.rcpjournals.org/content/23/2/144.full SO - Clin Med2023 Mar 01; 23 AB - Background Lateral flow tests (LFT) are point-of-care rapid antigen tests that allow isolation and control of disease outbreaks through convenient, practical testing. However, studies have shown significant variation in their diagnostic accuracy. We conducted a systematic review of the diagnostic accuracy of LFTs for the detection of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) to identify potential factors affecting their performance.Methods A systematic search of online databases was carried out to identify studies assessing the sensitivity and specificity of LFTs compared with polymerase chain reaction (PCR) tests. Data were extracted and used to calculate pooled sensitivity and specificity. Meta-regression analysis was conducted to identify covariates influencing diagnostic accuracy.Results In total, 76 articles with 108,820 test results were identified for analysis. Pooled sensitivity and specificity were 72% (95% confidence interval (CI): 0.68–0.76) and 100% (95% CI: 0.99–1.00), respectively. Staff operation of the LFT showed a statistically significant increase in sensitivity (p=0.04) and specificity (p=0.001) compared with self-operation by the test subjects. The use of LFTs in symptomatic patient subgroups also resulted in higher test sensitivity.Conclusion LFTs display good sensitivity and extremely good specificity for SARS-CoV-2 antigen detection; they become more sensitive in patients with symptoms and when performed by trained professionals.