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Serum peptidome profiling in patients with gastric cancer

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Abstract

To identify discriminating protein patterns in serum samples between gastric cancer patients (early and advanced stages) and healthy controls. We used magnetic bead-based separation followed by matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) to identify patients with gastric cancer. In total, serum samples from 62 gastric cancer patients (32 in the training set and 30 in the test set; 19 of which had early-stage tumors and 43 of which had advanced-stage tumors) and 64 healthy controls (32 in the training set and 32 in the test set) were analyzed. The mass spectra, analyzed using ClinProTools software, distinguished between cancer patients and healthy individuals based on three different algorithm models. In the training set, patients with gastric cancer could be identified with a mean sensitivity of 94.7% and a mean specificity of 99%. Similar results were obtained with the test set, showing 79.3% sensitivity and 86.5% specificity. Our study demonstrates the high sensitivity and specificity of screening serum protein patterns using MALDI-TOF MS for the identification of patients with gastric cancer.

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Acknowledgments

This work was supported by the National Science Foundation for Postdoctoral Scientists of China (Grant number: 20090461301); the Young Scientist Foundation from the Medical School in Xi’an Jiaotong University (Grant number: YQN0809); the Scientific Research Support Program for New Teachers (Grant number: 0116-081410-05); the Postdoctoral Science Foundation of Xi’an Jiaotong University (Grant number: 2116-04212217); Guang Hua Medical Innovation Research Foundation (Grant number: 0203407); and the International Sci-tech Cooperation Program (Grant number: 2009DFA31420).

Conflicts of interest

The authors confirm that they have no financial or personal relationships that cause a conflict of interest regarding the work in the manuscript.

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Authors and Affiliations

  1. Department of Genetics and Molecular Biology, Medical School of Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China

    Juan Yang, Tu-Sheng Song & Chen Huang

  2. Key Laboratory of Environment and Disease-Related Gene, Ministry of Education, 76 Western Yanta Road, Xi’an, 710061, Shaanxi, People’s Republic of China

    Juan Yang, Tu-Sheng Song & Chen Huang

  3. Department of Tumor Surgery, the First Affiliated Hospital, Xi’an Jiaotong University College of Medicine, 277 Western Yanta Road, Xi’an, 710004, Shaanxi, People’s Republic of China

    Yong-Chun Song, Cheng-Xue Dang & Zhi-Gang Liu

  4. Department of Medical Image, the First Affiliated Hospital of Xi’an Jiaotong University, 277 Western Yanta Road, Xi’an, 710004, Shaanxi, People’s Republic of China

    You-Min Guo

  5. Department of Respiratory Medicine, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China

    Zong-Fang Li

  6. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, 157 Xi Wu Road, Xi’an, 710004, Shaanxi, People’s Republic of China

    Zong-Fang Li

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  1. Juan Yang
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Correspondence to Chen Huang.

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Yang, J., Song, YC., Dang, CX. et al. Serum peptidome profiling in patients with gastric cancer. Clin Exp Med 12, 79–87 (2012). https://doi.org/10.1007/s10238-011-0149-2

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  • DOI: https://doi.org/10.1007/s10238-011-0149-2

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