Effect of low-fat, high-carbohydrate, high-fiber diet on fecal bile acids and neutral sterols

doi:10.1016/0091-7435(88)90042-4

Preventive Medicine

Volume 17, Issue 4, July 1988, Pages 432-439

https://doi.org/10.1016/0091-7435(88)90042-4 Get rights and content

Abstract

The effect of a diet low in total fat and high in complex carbohydrates on the excretion of bile acids and neutral sterols and on serum lipids was studied in women, 46–47 years old, who were consuming a mixed Western diet. Participants kept an initial 3-day food record while consuming their normal diet (pre-diet period). During the dietary intervention period (experimental diet) which lasted for 26 days, all volunteers consumed a low-calorie, low-fat (< 10% of total calories), high-fiber (37 g/day), high-carbohydrate diet. At the 1-year follow-up, the participants completed another 3-day food record, which indicates that these volunteers maintained their caloric and fat intake at levels slightly higher than the experimental diet, but lower than the pre-diet period. Individual 24-hr fecal samples for 2 days and blood samples were collected from the volunteers during each dietary period. Fecal samples were analyzed for neutral sterols and bile acids, and blood samples were analyzed to ascertain cholesterol and triglyceride levels. There were no significant differences in the excretion of neutral sterols between the dietary periods. Fecal secondary bile acids were significantly lower during the experimental and follow-up diet periods compared with the pre-test diet period. Serum cholesterol levels were significantly lower during the experimental and follow-up diet periods than during the pre-test diet period. These results suggest that switching from a high-fat, low-fiber diet to a low-fat, high-fiber diet can reduce the excretion of bile acids which are thought to be involved in the promotion of colon cancer.

References (36)

P. Correa et al.
Epidemiology of large bowel cancer
Adv. Cancer Res.
(1978)
J.H. Cummings et al.
Changes in fecal composition and colonic function due to cereal fiber
Amer. J. Clin. Nutr.
(1976)
H. Danielsson
Present status of research on metabolism and excretion of cholesterol
Adv. Lipid Res.
(1963)
M.J. Hill
Steroid nuclear dehydrogenation and colon cancer
Amer. J. Clin. Nutr.
(1974)
M.J. Hill et al.
Bacteria and etiology of cancer of the large bowel
Lancet
(1971)
R.M. Kay et al.
Effect of citrus pectin on blood lipids and fecal steroid excretion in man
Amer. J. Clin. Nutr.
(1977)
B.S. Reddy et al.
Effect of dietary wheat bran, alfalfa, pectin and carrageenan on plasma cholesterol and fecal bile acid and neutral sterol excretion in rats
J. Nutr.
(1980)
C.F. Adams
Nutritive Value of American Foods in Common Units
J.W. Anderson et al.
Plant Fiber and Food
(1980)
B. Armstrong et al.
Environmental factors and cancer incidence and mortality in different countries with special reference to dietary patterns
Int. J. Cancer
(1975)

J. Avigan et al.

Steroid and bile acids excretion in man and the effect of dietary fat

J. Clin. Invest.

(1965)

Barnard, R. J., Guzy, P. M., Rosenberg, J. M., and O'Brien, L. T. Effects of an intensive exercise and nutrition...

R.J. Barnard et al.

Response of non-insulin-dependent diabetes mellitus patients to the Pritikin program of diet and exercise

Diabetes Care

(1982)

R.J. Barnard et al.

Effects of an intensive, short-term exercise and nutrition program on patients with coronary heart disease

J. Cardiac Rehab.

(1981)

A.W. Bull et al.

The promotion of azoxymethane-induced intestinal cancer by highfat diet in rats

Cancer Res.

(1979)

D.P. Burkitt

Large bowel cancer

W.E. Connor et al.

Effect of dietary cholesterol upon serum lipids in man

J. Lab. Clin. Med.

(1961)

J.H. Cummings

Consequences of the metabolism of fiber in the human large intestine

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General articleEffect of low-fat, high-carbohydrate, high-fiber diet on fecal bile acids and neutral sterols

Abstract

Adv. Cancer Res.

Amer. J. Clin. Nutr.

Adv. Lipid Res.

Amer. J. Clin. Nutr.

Lancet

Amer. J. Clin. Nutr.

J. Nutr.

Nutritive Value of American Foods in Common Units

Plant Fiber and Food

Environmental factors and cancer incidence and mortality in different countries with special reference to dietary patterns

Int. J. Cancer

Steroid and bile acids excretion in man and the effect of dietary fat

J. Clin. Invest.

Response of non-insulin-dependent diabetes mellitus patients to the Pritikin program of diet and exercise

Diabetes Care

Effects of an intensive, short-term exercise and nutrition program on patients with coronary heart disease

J. Cardiac Rehab.

The promotion of azoxymethane-induced intestinal cancer by highfat diet in rats

Cancer Res.

Large bowel cancer

Effect of dietary cholesterol upon serum lipids in man

J. Lab. Clin. Med.

Consequences of the metabolism of fiber in the human large intestine

General article
Effect of low-fat, high-carbohydrate, high-fiber diet on fecal bile acids and neutral sterols