Regular ArticleProspective evaluation of environmental contamination by Clostridium difficile in isolation side rooms
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2024, Journal of Hospital InfectionRole of Clostridioides difficile in hospital environment and healthcare workers
2020, AnaerobeCitation Excerpt :Also, the enrichment broths have no germinant and this may influence the growth rate. Similarly, Verity et al. [15], observed a better recovery of C. difficile from swabs directly placed on plates containing lysozyme than from swabs processed with an enrichment broth step. In conclusion, by using a simplified method for laboratory processing of the Polywipes sponges, which yielded the most efficient recovery of C. difficile from environmental surfaces, we found a significant proportion of environmental hospital surfaces contaminated with toxigenic C. difficile as well as hand colonization of healthcare workers.
Approaches to the detection of Clostridioides difficile in the healthcare environment
2019, Journal of Hospital InfectionCitation Excerpt :This contrasts with a study by Verity et al. who compared direct inoculation on to cycloserine cefoxitin agar supplemented with 5 mg/L of lysozyme to inoculation on to the same agar following enrichment with Robertson's cooked media. They found that C. difficile recovery was significantly greater using the direct inoculation method compared to enrichment (137 positive samples vs 61 samples) [22]. The duration of incubation of culture plates varies across the studies reviewed.
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Author for correspondence: Dr M. H. Wilcox, Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK. Tel: +44 113 233 5596; Fax: +44 113 233 5649; E-mail:[email protected]