Energy metabolism of the inner cell mass and trophectoderm of the mouse blastocyst

doi:10.1111/j.1432-0436.2006.00052.x

Differentiation

Volume 74, Issue 1, February 2006, Pages 11-18

https://doi.org/10.1111/j.1432-0436.2006.00052.x Get rights and content

Abstract

Mammalian pre-implantation development culminates in the formation of the blastocyst consisting of two distinct cell lineages, approximately a third of the cells comprise the pluripotent inner cell mass (ICM) and the remainder the differentiated trophectoderm (TE). However, the contribution made by these two cell types to the overall energy metabolism of the intact blastocyst has received relatively little attention. In this study, the metabolism of the intact mouse blastocyst and isolated ICMs were determined in terms of total ATP formation (calculated from oxygen consumption and lactate formation), mitochondrial distribution and amino acid turnover to provide an indication of protein synthesis. The TE consumed significantly more oxygen, produced more ATP and contained a greater number of mitochondria than the ICM. Amino acid turnover was significantly greater (p<0.001) in the TE compared with the ICM. Specifically, there was a significant difference in the utilization of aspartate (p=0.020), glutamate (p=0.024), methionine (p=0.037), and serine (p=0.041) between the cells of the ICM and TE. These data suggest that the TE produces approximately 80% of the ATP generated and is responsible for 90% of amino acid turnover compared with the ICM. The major fate of the energy produced by the TE is likely to be the Na⁺, K⁺ATPase (sodium pump enzyme) located on the TE basolateral membrane. In conclusion, the pluripotent cells of the ICM display a relatively quiescent metabolism in comparison with that of the TE.

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ORIGINAL ARTICLEEnergy metabolism of the inner cell mass and trophectoderm of the mouse blastocyst

Abstract

FEBS Lett

FEBS Lett

Fertil Steril

Dev Biol

Arch Biochem Biophys

Dev Biol

Eur J Obstet Gynecol Reprod Biol

Theriogenology

FEBS Lett

Biochim Biophys Acta

Theriogenology

Arch Biochem Biophys

Biochem Biophys Res Commun

Theriogenology

Theriogenology

Regulation of hamster embryo development in vitro by amino acids

Polyamines inhibit apoptosis in porcine parthenotes developing in vitro

Mol Reprod Dev

Osmolarity dependent glycine accumulation indicates a role for glycine as an organic osmolyte in early preimplantation mouse embryos

Biol Reprod

Amino acids promote human blastocyst development in vitro

Hum Reprod

Can embryo metabolism be used fro selecting bovine embryos before transfer?

Reprod Nutr Dev

Taurine acts as an osmolyte in human and mouse oocytes and embryos

Biol Reprod

Amino acids and ammonium regulate mouse embryo development in culture

Biol Reprod

Non-invasive measurement of nutrient uptake by single cultured pre-implantation mouse embryos

Hum Reprod

Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro

J Reprod Fertil

Metabolic characterization of the bovine blastocyst, inner cell mass, trophectoderm and blastocoel fluid

Reproduction

Contribution by different fuels and metabolic pathways to the total ATP turnover of proliferating MCF-7 breast cancer cells

Biochem J

Energy metabolism of the trophectoderm and inner cell mass of the mouse blastocyst

J Exp Zool

Ultrastructural and autoradiographic studies of mouse cleavage stages

Am J Anat

ORIGINAL ARTICLE
Energy metabolism of the inner cell mass and trophectoderm of the mouse blastocyst