Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion

Cell. 1991 Nov 29;67(5):869-77. doi: 10.1016/0092-8674(91)90360-b.

Abstract

Pemphigus vulgaris (PV) is a life-threatening skin disease in which autoantibodies against a keratinocyte cell surface 130 kd glycoprotein, PV antigen (PVA), cause loss of cell-cell adhesion, with resultant epidermal blisters. We used affinity-purified PV IgG to isolate cDNA, containing the entire coding sequence for PVA, from human keratinocyte expression libraries. Northern blot analysis indicated PV mRNA expression only in stratified squamous epithelia. The deduced amino acid sequence of PVA was unique but showed significant homology with members of the cadherin family of Ca(2+)-dependent cell adhesion molecules, most markedly to desmoglein I. These findings demonstrate that a novel epithelial cadherin is the target of autoantibodies in PV.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Autoantibodies / immunology*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cadherins / genetics
  • Cadherins / immunology*
  • Cell Adhesion
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Desmoglein 3
  • Epithelium / immunology
  • Gene Library
  • Humans
  • Immunoblotting
  • Immunoglobulin G / immunology*
  • Keratinocytes / immunology
  • Molecular Sequence Data
  • Pemphigus / genetics
  • Pemphigus / immunology*
  • Poly A / genetics
  • Poly A / isolation & purification
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid

Substances

  • Autoantibodies
  • Cadherins
  • DSG3 protein, human
  • Desmoglein 3
  • Immunoglobulin G
  • RNA, Messenger
  • Poly A
  • RNA
  • DNA

Associated data

  • GENBANK/M76482
  • GENBANK/S60911
  • GENBANK/S60914
  • GENBANK/S60917
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S60969
  • GENBANK/S61524
  • GENBANK/S66441
  • GENBANK/S66442