Multiple base-recognition sites in a biological nanopore: two heads are better than one

David Stoddart; Giovanni Maglia; Ellina Mikhailova; Andrew J Heron; Hagan Bayley

doi:10.1002/anie.200905483

Multiple base-recognition sites in a biological nanopore: two heads are better than one

Angew Chem Int Ed Engl. 2010;49(3):556-9. doi: 10.1002/anie.200905483.

Authors

David Stoddart¹, Giovanni Maglia, Ellina Mikhailova, Andrew J Heron, Hagan Bayley

Affiliation

¹ Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, OX1 3TA, UK.

Abstract

Ultra-rapid sequencing of DNA strands with nanopores is under intense investigation. The αHL protein nanopore is a leading candidate sensor for this approach. Multiple base-recognition sites have been identified in engineered αHL pores. By using immobilized synthetic oligonucleotides, we show here that additional sequence information can be gained when two recognition sites, rather than one, are employed within a single nanopore.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins
Base Sequence
Biotin / analogs & derivatives
Hemolysin Proteins / chemistry*
Models, Biological*
Molecular Sequence Data
Nanostructures / chemistry*
Porosity
Sequence Analysis, DNA / methods*

Substances

Bacterial Proteins
Hemolysin Proteins
biotin-streptavidin complex
Biotin

Abstract

Publication types

MeSH terms

Substances

Grants and funding