User profiles for John Alcolado

John Alcolado

Verified email at chester.ac.uk
Cited by 2393

Mitochondrial DNA defects: a widening clinical spectrum of disorders.

EJ Sherratt, AW Thomas, JC Alcolado - Clinical Science (London …, 1997 - europepmc.org
1. Mitochondrial DNA has a number of interesting properties including maternal transmission,
the ability to replicate in post-mitotic cells, a high mutation rate and an extremely compact …

Genetics of diabetic complications

J Alcolado - The Lancet, 1998 - thelancet.com
In 1965 Neel described diabetes mellitus as the “geneticist’s nightmare”. 1 Twin, family, and
transracial studies suggested that inherited factors were important in the aetiology of the …

Nurse practitioners and the future of general practice

J Alcolado - BMJ, 2000 - bmj.com
In my daily clinical practice I work closely and happily with nurses who specialise in looking
after patients with diabetes. An increasing number of tasks, previously the domain of doctors, …

The detection of mitochondrial DNA mutations using single stranded conformation polymorphism (SSCP) analysis and heteroduplex analysis

…, R Morgan, M Sweeney, A Rees, J Alcolado - Human genetics, 1994 - Springer
A number of mitochondrial (mt) point mutations have been associated with inherited
disorders. These pathogenic mutations are usually heteroplasmic. Here we describe the …

Mitochondrial DNA variations in patients with Type 2 (non‐insulin dependent) diabetes mellitus and a Welsh control population

…, AW Thomas, JW Gagg, A Majid, JC Alcolado - Human …, 1999 - Wiley Online Library
Type 2 (non‐insulin dependent) diabetes mellitus may be inherited along the maternal line
and a variety of mitochondrial DNA (mtDNA) variants have been implicated in the …

DNA polymorphisms as disease markers

DJ Galton, JC Alcolado - DNA Polymorphisms as Disease Markers, 1991 - Springer
One of the surprizing observations arising from recent sequencing studies of the human
genome is the large amount of nucleotide variation that is found in intergenic and intronic DNA. …

Genetic linkage study of a major susceptibility locus (D2S125) in a British population of non-insulin dependent diabetic sib-pairs using a simple non-isotopic screening …

…, JW Gagg, SA Davies, M Aneela, JC Alcolado - Human genetics, 1997 - Springer
A polymorphic microsatellite marker (D2S125) was recently reported to show significant
linkage to non-insulin dependent diabetes mellitus (NIDDM) in a population of Mexican-…

Phylogenetic analysis of mitochondrial DNA in type 2 diabetes: maternal history and ancient population expansion.

…, AW Thomas, R Gill-Randall, JC Alcolado - Diabetes, 1999 - Am Diabetes Assoc
Several studies have suggested a maternal excess in the transmission of type 2 (non-insulin-dependent)
diabetes. However, the majority of these reports rely on patients recalling …

Nonradioactive characterization of low-level heteroplasmic mitochondrial DNA mutations by SSCP-PCR enrichment

EJ Sherratt, AW Thomas, JW Gagg, JC Alcolado - Biotechniques, 1996 - Future Science
Mitochondrial DNA (mtDNA) mutations have been implicated in an increasing number of
human diseases. Many of these mutations are heteroplasmic and are only present at low levels …

[PDF][PDF] CSII therapy: the Cardiff experience

J Alcolado, K Bailey, S Cuthbert… - Diabetes and Primary …, 2008 - diabetesonthenet.com
The University Hospital of Wales in Cardiff is the major teaching hospital in Wales and
together with Llandough Hospital in the East of the City, provides diabetes services for a …